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LC-MALDI | AccuSpot | Overview of the technology
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Bringing LC to MALDI technology
Delivers increased sequence coverage

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Overview of the technology
  • The AccuSpot uses a unique sample spotting method. It is simply called the 'Half contact method' designed to precisely control the volume of the droplet delivered to the plate. In this way the ‘collection plate’ is moved up to the static LC probe at a specific time (the set time depends on flow rate and the volume of LC spots required for transfer).
  • The volume of spots applied to the sample plate is precisely controlled between 10 nL to 1,000 nL (time-specific mode). In addition, the matrix solution flow rate can be applied at rates between 0.1µL/min to 10 µL/min.
  • To accommodate different needs the spotting direction can be either straight or zigzag.
  • To ensure that the droplet formation and collection is consistent a CCD camera is used to monitor micro-fractionaction.
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Step 1
Droplet formation. Capillary position is static whilst the MALDI plate is the standby-position.
Droplet Formation
Step 2
Drop deposition. The MALDI plate is precisely moved to the droplet.
Droplet Deposition
Step 3
Reset. The MALDI plate returns to the standby position
MALDI plate returns to standby

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