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PG220 gel image analysis Overview
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Includes Cross Stain Analysis for the analysis of multiplexed gels
enabling direct comparisons between groups of images
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PG220 includes CSA (Cross Stain Analysis) support to work with multiplexed gels that include an internal standard (such as different fluorescence labels).

Key benefits and features:-
  • Protein expression comparisons between 2D gels is not only time-consuming it is also highly complex. However, one approach that has been used to differentiate between samples is to consider the application of different dyes to the gel. Each stain is applied to the sample and spots are specifically detected at different wavelengths.
  • Cross stain analysis (CSA) supports multiplexed gel analysis and differential fluorescent staining protocols as well as Sypro. It is a highly advanced technology platform that will meet current and future needs in gel image analysis software.
  
Dye 3
(550nm)
Dye 2
(480nm)
Dye 5
(630nm)

Cross stain analysis (CSA) begins with two protein samples that may differ in composition. These are each stained with a fluorescent dye, samples are pooled and then separated on the same 2D gel. Images from this experiment are acquired at different wavelengths. The technique produces almost identical separation between samples. An internal standard dye can be used for reference purposes.
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