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BioSpec-mini Nucleic acid quantitation mode
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Comprehensive quantitation tools for nucleic acids
Including Oligo and Warburg-Christian quantitation modes
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  • Nucleic acid quantitation mode.
    Simple and robust quantitation mode uses 260nm absorbance and preset coefficient factors for dsDNA, ssDNA, oligoDNA and RNA.
  • Oligo quantitation mode.
    Used to quantify the primer and several tens of mer single-stranded DNA/RNA. Input the base composition (LongOligo quantitation) and base sequence (ShortOligo quantitation) to automatically calculate the molecular weight and molar absorbance coefficient. Calculated parameters are automatically used for quantitation from absorbance at 260nm.
  • Warburg-Christian quantitation mode.
    This is appropriate for samples containing both proteins and nucleic acids. This mode can be used to determine the total amount of nucleic acid by subtracting protein interference concentration.

    Spectrum measurement (fixed between 190nm and 330nm) is possible before performing quantitation calculations, so impurities and admixture can be easily checked on the large LCD.
  • One-touch quantitation.
    Just press a button at the spectrum screen to enable quantitation calculating.
  • Internal background correction.
    Absorbance at each wavelength (230nm, 260nm and 280nm) is subtracted by the absorbance at the background wavelength (default 320nm) prior to calculation of absorbance ratio and concentration.
  • Optical path length and dilution ratio corrections are available.
  • Nucleic acid purity.
    It can be checked because the A260/A280nm and A260/A230nm absorbance ratios are displayed on the concentration calculation screen together with the sample concentration and absorbance values.
Next - Tm prediction mode and protein quantitation mode  >> 
Spectrum mode and Bacteria counting mode  >> 

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