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BioSpec-mini Tm prediction and protein quantitation mode
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Melting temperature (Tm) mode
Protein quantitation methods (Lowry, BCA, Bradford and Biuret)
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- Tm prediction mode.
Tm (melting temperature) is the temperature taken at mid-melting point when the temperature is raised to dissociate the double-stranded DNA or RNA into single strands. The higher the Tm, the more stable the double-strand structure. Generally, Tm becomes higher with the increase in the number of the GC pairs. Tm value is an important parameter for PCR.
Simple Tm prediction. Tm for double-strand DNA is estimated using the following equation.
- Tm estimation value = 81.5 + 16.6* log
- (salt concentration) - 675/(strand length) + 0.41* (%GC)
- Calculation by the nearest neighbor base pair model.
Tm and thermodynamic parameters (ΔG, ΔH and ΔS) for double-strand formations of DNA/DNA and RNA/RNA duplex can be calculated by inputting the base sequence. This results in a higher accuracy relative to the simple Tm prediction.
- Protein quantitation mode.
- The package consists of four quantitation methods (Lowry, BCA, Bradford and Biuret) using coloring reagents to measure protein concentration, and a UV absorption method that calculates concentration from absorbance at 280nm.
- Multiple point calibration (up to 10 points) and 3 types of calibration curves ( linear, quadratic and cubic equations) are supported.
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